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Week 2: Identifying an Unknown Antibiotic Resistance Gene

How to GroW (Almost) Anything 2020

Step 0: Prelab questions

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Step 1: Restriction Digest to generate a ladder

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Step 2: Isolation of genomic DNA from bacteria

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Step 4: Repairing and cloning the DNA library into plasmids for sequencing

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Step 5: Identification of the Spectinomycin Resistance Cassette

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