Week 2
by BELEN VICENTE
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Week 2: Identifying an Unknown Antibiotic Resistance Gene
How to GroW (Almost) Anything 2020
Step 0: Prelab questions
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Step 1: Restriction Digest to generate a ladder
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Step 2: Isolation of genomic DNA from bacteria
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Step 3: Shearing of the genomic DNA to create a DNA fragment library
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Step 4: Repairing and cloning the DNA library into plasmids for sequencing
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Step 5: Identification of the Spectinomycin Resistance Cassette
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