General takeaways:

1. Pluronic v.s. Cells concentration / ratio:
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‍It was important to get the ratio right, since too little pluronic will cause the bioink unable to reach a semi-solid, printable state, while too much will cause uneven deposition and potential clotting. The optimal we found was a 3:1 ratio. The process of mixing was hard. Our team learned that we must first fill the pipettes with more than enough pluronic at its liquid state so there will still be enough when depositing it into the tube (as some of the liquid will cling onto the walls). Afterwards, as long as we mix the cells in carefully without creating bubbles, the print should be successful.

2. CAD and Setup

Design constraints
- Can't be too high
- Can't have intricate details
- Can't do cantilever structures
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Printing Setup
- In order to print successfully, the printer must be properly zeroed & the layer height should be carefully calculated.