How to Grow Almost Anything 2021

Principles & Practices Assignments

Class Recording
Recitation Recording

Ethics, safety and security are essential considerations throughout (and beyond!) this class. We have therefore designed an assignment this week to give you a strong foundation, and then will ask you to reflect each week and in the design of your final project.

Principles & Practices -  Class 1 Assignment

This is one framework but feel free to make your own

Your context:

Does the option

Option 1:

Option 2:

Option 3:

Enhance Biosecurity

  • By preventing incidents

  • By helping respond

Foster Lab Safety

  • By preventing incident

  • By helping respond

Protect the environment

  • By preventing incidents

  • By helping respond

Other considerations

  • Minimizing costs and burdens to stakeholders

  • Feasibility?

  • Not impede research

  • Promote constructive applications

Principles & Practices - Weekly Homework

Principles & Practices - Final Project Homework

Related Readings and Resources (Palmer)

Related Readings and Resources (Church, Jacobson, Kong)

Comprehensive AAV capsid fitness landscape reveals a viral gene and enables machine-guided design (Ogden, Kelsic, 2019)

Next-Generation Digital Information Storage in DNA (Church, Gao, Kosuri, 2012)

Rapidly evolving homing CRISPR barcodes (Kalhor, Mali, Church 2016)

Developmental barcoding of whole mouse via homing CRISPR (Kalhor, Mali, Church 2018)

Toward eliminating HLA class I expression to generate universal cells from allogeneic donors | Blood (ZFN: Torikai 2013)

A Multidrug-resistant Engineered CAR T Cell for Allogeneic Combination Immunotherapy (TALEN: Valton ... Poirot 2015- Cellectis)

CRISPR-Cas9-mediated multiplex gene editing in CAR-T cells (CRISPR: Yang, et al 2015)

Genome-wide inactivation of porcine endogenous retroviruses (PERVs) (CRISPR: Yang, et al 2015)

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9 (Niu, et al. 2017)

Full article: Harnessing gene drive (Min, Smidler, Najjar, Esvelt 2017)

Review The Hallmarks of Aging (Carlos López-Otín, et al. 2013)

A single combination gene therapy treats multiple age-related diseases (Noah Davidsohn et al. 2019)