HTGAA : 3D Bio Printing & Biofabrication

Dr. Nina Tandon (Epibone) and Filippos Tourlomousis (Center for Bits and Atoms, MIT)

Bioprinting Workflow

Task 1: Engineered Cells

For example perform a transformation protocol in order to produce Ecoli bacteria that express GFP.

Task 2: Bioink Fomrulation

We are going to use a thermoresponsive gel called Pluronic .Here the task is to learn how to optimize its rheological properties for optimum printability with and without cells:

Task 3: Printing using Direct Writing Microextrusion

After having optimized your bioink formulation, design and print whatever you want using a custom-made bioprinter. We are going to learn about bioprinting design rules. Printing 3D soft matter structures with well defined geometrical features requires a careful parametric study of the independent process parameters (extrusion rate, tranlational stage speed are the most important ones considering that you have optimized the ink's rheological properties).

Task 4: Characterize your bioprinted construct