Week 8
Synthetic Minimal Cells
GFP is green fluorescent protein – you can go learn more on Wikipedia. Mature GFP is fluorescent on it’s own – the fluorophore is made of amino acids from the GFP protein itself.
FIAsH is a very short protein, not fluorescent on its own. The fluorescence comes from the ligand, a small molecule that’s bound to the FIAsH peptide. The ligand’s full name is fluorescein arsenical helix binder bis-EDT adduct, FIAsH-EDT2.