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Final Project


This page is for tracking and understanding the final project I have been trying to complete. I will upload a alot of paper calculations but once the fully speculative part of the project is done, I will probaly try and addionally upload things to gitlab.

Characterising Dinoflagellites against other sources of Luminescence


Over the next couple of calculations I will attempt to characterise the micro organism by the name of dinoflagellite against other sources of light. Mostly inspired by the Noise and Optics chapters of the course.

Prior to the calculations I have also attempted to observe the organism in a low-cost field spectrometer, which was semi successfull. In the days following the final, now that I am back in the lab physically I would like to make adjustment to the low-cost spectrometer so it is more suitable to documenting the observations made through it.
As for now observations were only possible with the human eye. This was however good enough to confirm the wavelength as 475nm. in the blue/green spectrum. Actually it was very intersting to observe as it was very nearly pure.

Video Documentation

Here are some video recordings of the observations:



Image Documentation

Here are the calculations:

Observation 0

Observation 1

Observation 2

Observation 3

Observation 4
On this last calculation, I started with the assumption of a quantum yield of 1 based on some literature regarding the chemical reaction which seemed to suggest that one luciferase reaction resulted in one photon. But there was another paper since, which suggested a more realistic 0.3 (30percent) efficiency rate.

Observation 5

Observation 6

Observation 7

Observation 8

Following these calculation the next intersting step would be to measure the amount of photons myself using a photodiode.

Speculative experiment


Based on these calculations, I would like to suggest a speculative experiment.
Observation 9










References


Getting some of this to work, required some key reading references:
Crystal structure of a pH-regulated luciferase catalyzing the bioluminescent oxidation of an open tetrapyrrole

Bioluminescence – The Vibrant Glow of Nature and its Chemical Mechanisms

The ultrastructural localization of luciferase in three bioluminescent dinoflagellates, two species of Pyrocystis, and Noctiluca, using anti-luciferase and immunogold labelling