In this lab we tested the impact temperature and growth media composition has on the production of lycopene in E coli.
Lycopene: the red pigment that gives tomatoes their color. "Bright red carotene and carotenoid pigment and phytochemical found in tomatoes and other red fruits and vegetables, such as red carrots, watermelons, etc."
Turpene: "any of a large group of volatile unsaturated hydrocarbons found in the essential oils of plants, especially conifers and citrus trees. They are based on a cyclic molecule having the formula C10H16."
Beta-barrel: "A beta barrel is a beta-sheet composed of tandem repeats that twists and coils to form a closed toroidal structure in which the first strand is bonded to the last strand (hydrogen bond)."
Chromophore: "an atom or group whose presence is responsible for the color of a compound."
|Plasmid Content||Growth Media||Growth Temperature|
Q. Using tools such as Biocyc and KEGG, review enzymes that can be added to (or knocked out of) your lycopene-producing E. coli to increase the amount of lycopene they produce. Compare the expected and experimental differences in production efficiency between bacteria that carry the pAC-LYC and pAC-LYCipi plasmids.
A. Looking at the Carotenoid biosynthesis pathway from KEGG it looks like there are quite a few enzymes that could be added to boost production, including phytoene, phytofluene, Z-carotene, etc.
Q. Using Biocyc and/or KEGG, can you identify the enzyme to convert the carrot-orange pigment beta-carotene to the corn-yellow pigment zeaxanthin?
A. CrtT and LUT5 convert B-carotene to B-cryptoxanthin and then to zeaxanthin